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Solvent and solid-phase extraction of natural and synthetic corticoids in human urine

A. Santos-Montes, R. Gonzalo-Lumbreras, A.I. Gasco-Lopez, R. Izquierdo-Hornillos

Optimization of the main variables that affect solvent and solid-phase extraction processes, using disposable C18 cartridges and the non-ionic polymeric resin Serdolit AD-2, of human urine containing natural and synthetic corticoids is described. The data were obtained from different HPLC separations of these compounds using calibration graphs obtained before and after extraction of these compounds. The procedures, including sample preconcentration, showed efficiencies over 90\%. NaCl was used to avoid emulsion formation in solvent extraction. The results achieved using solvent and solid-phase extraction are discussed.


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Journal of Chromatography B. Biomedical applications. Volumen: 652 Numero: 1 Páginas: 83-9

Índice de impacto JCR y cuartil Scopus: JCR impact factor: 1.911 (2001)

Referencia DOI: DOI icon 10.1016/0378-4347(93)E0396-8    

Publicado en papel: Enero 1994.



Cita:
A. Santos-Montes, R. Gonzalo-Lumbreras, A.I. Gasco-Lopez, R. Izquierdo-Hornillos. Solvent and solid-phase extraction of natural and synthetic corticoids in human urine. Journal of Chromatography B. Biomedical applications. vol. 652, no. 1, pp. 83-9, Enero 1994.


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